Degree Name

Master of Science (MS)

Semester of Degree Completion

1972

Thesis Director

Joan F. White

Abstract

In order to obtain proper muscle development in tissue culture, certain conditions must prevail. There is a delicate balance of factors, and elimination of even one of these factors leads to poor and improper development. Muscle cells grown in the absence of fibroblasts usually fail to differentiate. Therefore, proper development depends upon the presence of two cell types: myoblasts and fibroblasts. Investigators have demonstrated that the fibroblasts are necessary in that they produce collagen which is necessary for proper muscle development. The present study has shown that desoxycorticosterone, hydrocortisone, and prednisone at concentrations of 10, 1, and 0.1 ug/ml of culture medium alter muscle development to varying degrees. Hydrocortisone has proven to be the most inhibitory steroid. Desoxycorticosterone treated cultures have yielded very poor muscle development at 10 ug/ml and allowed development to a contracting state at 0.1 ug/ml. Prednisone treated cultures have developed the best at all three concentrations.

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