Degree Name

Master of Science (MS)

Semester of Degree Completion

1987

Thesis Director

Russell Carlson

Abstract

The LPS of R. leguminosarum 128C53 smr rifr (a streptomycin and rifampicin resistant strain of wild type 128C53) and its mutant ANU54 (nod-, fix-) were isolated from the bacterial pellet by hot phenol/water extraction followed by gel filtration chromatography. The O-antigen was isolated by mild-acid hydrolysis of the LPS and purified by gel filtration chromatography using a Sephadex G-50 or G-25. The following results were the same for both the parent and mutant. The composition and linkage of the O-antigen were determined by gas chromatography (GC), GC-mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy (NMR) techniques. The data indicate that the O-antigen of the LPS from parent R. leguminosarum 128C53 and its mutant ANU54 are identical. The O-antigen contains a tetrasaccharide repeating unit. The backbone consists of one 1,3-linked-rhamnose and two 1,3-linked-fucose residues. A terminal mannose is linked to the 2-position of one of the two fucose residues. The 1H-NMR analysis indicates that all the glycosyl residues are alpha linked. The exact position of the mannose residue is under further investigation.

Download

Included in

Chemistry Commons

Share

COinS