Degree Name

Master of Science (MS)

Semester of Degree Completion

1979

Thesis Director

William A. Weiler

Abstract

The biodegradation of crude oil by bacteria was studied. Hydrocarbon-utilizing bacteria were isolated from sixteen brine pits from an oil producing region of Clark County, Illinois. The bacteria were capable of growth from the vapors arising from crude oil as a sole carbon source. Some strains of bacteria were capable of emulsifying crude oil, apparently through the production of an emulsifying agent(s) that was found to be heat stable and cell bound but which can be partially stripped from the cell by autoclaving. The bacteria appear to belong to the genus Arthrobacter. The chemical environment of the pits was examined and phosphorous levels were suggested as the possible limiting factor in the number of hydrocarbon-utilizing bacteria present.

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Microbiology Commons

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