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Undergraduate, 2nd Place: In Vitro Characterization of Fluorogenic Chemical Tools to Study Human Carboxylesterases (CESs)
Human carboxylesterases (CESs) are enzymes that are responsible for the metabolism of many important pharmaceuticals. Although CESs are key players in the hydrolysis of many ester-containing drugs, they remain understudied. Our group hypothesizes that this is primarily because there are few methods capable of reporting activity in live cells. Here, I report a new series of fluorogenic chemical tools to study the CES activity of one of the two major CESs in humans, CES1, in live cells. MCP-Me, MCP-Et, and MCP-iPr utilize the same carbonate group of a previously developed chemical tool in our group, FCP-1, and work to mimic the substrates of common drugs to study CES1 activity in vitro. By improving methods used to study CES1 activity, we can improve the individual efficacy of pharmaceuticals by ensuring prescribed drugs are compatible with the patient’s metabolic enzymes activity.
Spidle, Taylor, "Undergraduate, 2nd Place: In Vitro Characterization of Fluorogenic Chemical Tools to Study Human Carboxylesterases (CESs)" (2023). 2023 Awards for Excellence in Student Research and Creative Activity - Documents. 6.
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