Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease

Authors

Gary Fritz, Eastern Illinois UniversityFollow
C. L. Brelsfoard, Eastern Illinois University
R. Rodriguez, Escuela Te´cnica de Salud Boliviano Japonesa

Document Type

Article

Publication Date

July 2006

Abstract

Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.

Comments

'This article is the copyright property of the Entomological Society of America and may not be used for any commercial or other private purpose without specific written permission of the Entomological Society of America'

Recommended Citation

Fritz, Gary; Brelsfoard, C. L.; and Rodriguez, R., "Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease" (2006). Faculty Research & Creative Activity. 202.
https://thekeep.eiu.edu/bio_fac/202