Graduate Program
Biological Sciences
Degree Name
Master of Science (MS)
Semester of Degree Completion
Spring 2025
Thesis Director
Elliott Zieman
Thesis Committee Member
Antony O. Oluoch
Thesis Committee Member
Chris Stone
Abstract
The Asian tiger mosquito, Aedes albopictus, is a significant vector for diseases such as Dengue, Chikungunya, and Zika. Its increasing resistance to insecticides poses a critical challenge for vector control efforts. This study investigates knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) genes, specifically V1016G and F1534C, which are associated with pyrethroid resistance. Aedes albopictus populations from Central Illinois were analyzed using allele-specific PCR techniques to address three primary objectives: (1) determining whether V1016G and F1534C mutations are present in these populations, (2) determining which mutation is more widespread, and (3) investigating how resistance gene distribution differs between samples obtained in 2016-2017 and 2023-2024.
Allele-specific PCR revealed that none of the tested samples carried V1016G or F1534C mutations with the narrow Confidence Interval range prevalence of 3.7%; all individuals were wild type for these alleles. These findings indicate that Aedes albopictus populations in Central Illinois remain susceptible to pyrethroid insecticides.
This study underscores the importance of regular monitoring to detect emerging resistance mechanisms and guide local vector control strategies. By establishing a baseline of susceptibility, it highlights the opportunity to sustain effective use of pyrethroids in Central Illinois. while encouraging proactive surveillance to address potential future resistance.
Recommended Citation
Ochieng, Merylann Akinyi, "Prevalence of Insecticide Resistance Gene in Aedes albopictus Populations of Central Illinois" (2025). Masters Theses. 5063.
https://thekeep.eiu.edu/theses/5063
