Degree Name

Master of Science (MS)

Semester of Degree Completion


Thesis Director

William J. Keppler


Manometric, spectrophotometric, photomicrographic and quantitative biochemical techniques were employed to determine the morphological and physiological effects of 2,4-D on Gram-positive and Gram-negative bacteria.

The growth and motility of Gram-positive bacteria appeared to be more adversely affected than the growth and motility of Gram-negative species. The terminal oxidation, lysis, and chain length of both Gram-positive and Gram-negative bacteria did not appear to be affected by the concentrations used. The average cell size of both Gram-positive and Gram-negative bacteria was observed to increase after exposure to 1000 ppm 2,4-D. The normal leakage of amino acid and ultraviolet light absorbing cell constituents appeared to be retarded by 1000 ppm 2,4-D.