Degree Name

Master of Science (MS)

Semester of Degree Completion


Thesis Director

William A. Weiler


Effluent samples were taken at various stages of sewage treatment at a local wastewater treatment plant. Primary, secondary, tertiary and post-chlorinated effluents were sampled. The agar overlay method was used to determine the numbers of plaque-forming bacteriophage particles on thirty-six species of enteric bacteria from the Eastern Illinois University stock culture collection. Twenty-three pure culture phage isolates were prepared from selected plaques and their host range specificity was determined. For every host organism tested, a fifty percent or greater reduction of bacteriophage was evident through the sewage treatment process. Results obtained from investigation of host range specificity indicate that potential exists for phage-mediated transduction to occur in a natural environment.

Included in

Microbiology Commons