Master of Science (MS)
Semester of Degree Completion
Kandy D. Baumgardner
Water samples from the Charleston, Illinois water supply and selected agricultural run-off areas were tested for the presence of organic mutagenic chemicals. The Ames Salmonella/microsome mutagenicity assay utilizing strains TA98 and TA100 was used to analyze concentrated water samples. A special set of samples was exposed to the S9 activation system coupled with the TA98 strain to simulate the mammalian metabolic system.
No mutagens were detected in any of the samples tested. Several positive and negative control systems were included in the testing system. Negative control systems were used to determine the average spontaneous reversion rates for each strain for each test set. Positive control systems were utilized to demonstrate the ability of the strains to express positive results in the presence of known mutagens.
Washburn, Steven Lee, "Analysis of the Charleston, Illinois Water Supply for the Presence of Organic Mutagens Utilizing the Ames Salmonella/Microsome Assay" (1986). Masters Theses. 2735.