Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum

Authors

M. Dean Savage, University of Mississippi Main Campus
Zhongren Wu, University of Mississippi Main Campus
Steven L. Daniel, Eastern Illinois UniversityFollow
Leon L. Lundie JR, University of Mississippi Main Campus
Harold L. Drake, University of Mississippi Main Campus

Document Type

Article

Publication Date

August 1987

Abstract

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

Recommended Citation

Savage, M. Dean; Wu, Zhongren; Daniel, Steven L.; Lundie, Leon L. JR; and Drake, Harold L., "Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum" (1987). Faculty Research & Creative Activity. 135.
https://thekeep.eiu.edu/bio_fac/135